Hotspot

rpl36-infA

Genome · 79880-80471 · 592 bp

Back to Torreya

Pi 0.0155
MarkerSeek score 51.40
Primer available yes
Label rpl36-infA

Feature Metadata

label_name
rpl36-infA
feature_type
igs
region
Genome
length_bp
592
variable_sites
19
pi
0.0155
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
51.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl36-infA_p1 ACGAACAGAAGCACGGATTT ATGATGATGATGCTGATGTT 45.0 35.0 58.1 52.7 640-645 bp 1.0 43.7
rpl36-infA_p2 ACGAACAGAAGCACGGATTT TGATGATGATGCTGATGTTATCT 45.0 34.8 58.1 55.6 639-644 bp 1.0 43.7
rpl36-infA_p3 ACGAACAGAAGCACGGATTT TGATGATGATGCTGATGTTATCTT 45.0 33.3 58.1 56.3 639-644 bp 1.0 43.7
rpl36-infA_p4 TTTACGAACAGAAGCACGGA ATGATGATGATGCTGATGTT 45.0 35.0 57.5 52.7 643-648 bp 1.0 43.7
rpl36-infA_p5 TTTACGAACAGAAGCACGGA TGATGATGATGCTGATGTTATCT 45.0 34.8 57.5 55.6 642-647 bp 1.0 43.7