Hotspot

psbJ-petA

Genome · 67446-68426 · 981 bp

Back to Torreya

Pi 0.0089

MarkerSeek score 67.70

Primer available yes

Label psbJ-petA

Feature Metadata

label_name: psbJ-petA
feature_type: igs
region: Genome
length_bp: 981
variable_sites: 18
pi: 0.0089
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbJ-petA_p1	TCCGGATTAGGATCATCCCT	TAGCATCTGTCGTTTTGGCA	50.0	45.0	57.0	57.8	1078-1111 bp	1.0	73.4
psbJ-petA_p2	TCCGGATTAGGATCATCCCT	TCTGTCGTTTTGGCACAGAT	50.0	45.0	57.0	57.7	1073-1106 bp	1.0	72.8
psbJ-petA_p3	CGGAAGGATCCCTCTTTGGT	TAGCATCTGTCGTTTTGGCA	55.0	45.0	59.1	57.8	1163-1196 bp	1.0	72.7
psbJ-petA_p4	TCCGGATTAGGATCATCCCT	CATCTGTCGTTTTGGCACAG	50.0	50.0	57.0	57.7	1075-1108 bp	1.0	72.3
psbJ-petA_p5	CGGAAGGATCCCTCTTTGGT	TCTGTCGTTTTGGCACAGAT	55.0	45.0	59.1	57.7	1158-1191 bp	1.0	72.1