Hotspot

accD-rps12

Genome · 59900-61783 · 1884 bp

Back to Torreya

Pi 0.0108

MarkerSeek score 53.20

Primer available yes

Label accD-rps12

Feature Metadata

label_name: accD-rps12
feature_type: igs
region: Genome
length_bp: 1884
variable_sites: 40
pi: 0.0108
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 53.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-rps12_p1	TATCGTATGTGCTTCTGGCG	CCGTTTCAGCTTACGATCCA	50.0	50.0	57.9	58.0	2370-2597 bp	1.0	81.6
accD-rps12_p2	TTGGCTAACGTCACGATTGT	CCGTTTCAGCTTACGATCCA	45.0	50.0	57.8	58.0	2201-2428 bp	1.0	81.6
accD-rps12_p3	TGTTGGCTAACGTCACGATT	CCAATGGCTTTTGCTACTGC	45.0	50.0	57.8	58.0	2789-2958 bp	0.8	72.9
accD-rps12_p4	TTGGCTAACGTCACGATTGT	CCAATGGCTTTTGCTACTGC	45.0	50.0	57.8	58.0	2787-2956 bp	0.8	72.9
accD-rps12_p5	TATCGTATGTGCTTCTGGCG	CCAATGGCTTTTGCTACTGC	50.0	50.0	57.9	58.0	2956-2988 bp	0.6	64.5