Hotspot

trnF-GAA-ndhJ

Genome · 48079-48497 · 419 bp

Back to Torreya

Pi 0.0105

MarkerSeek score 55.00

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: Genome
length_bp: 419
variable_sites: 9
pi: 0.0105
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	AGGACTGAAAATCCTCGTGC	AAAGTTGGATTGGTTGGCCT	50.0	45.0	57.9	57.9	538-540 bp	1.0	80.3
trnF-GAA-ndhJ_p2	AGGACTGAAAATCCTCGTGC	AAGTTGGATTGGTTGGCCTT	50.0	45.0	57.9	57.9	537-539 bp	1.0	80.3
trnF-GAA-ndhJ_p3	AGGACTGAAAATCCTCGTGC	AGTTGGATTGGTTGGCCTTT	50.0	45.0	57.9	57.9	536-538 bp	1.0	80.3
trnF-GAA-ndhJ_p4	GACTGAAAATCCTCGTGCCA	AAGTTGGATTGGTTGGCCTT	50.0	45.0	58.2	57.9	535-537 bp	1.0	79.6
trnF-GAA-ndhJ_p5	GACTGAAAATCCTCGTGCCA	AGTTGGATTGGTTGGCCTTT	50.0	45.0	58.2	57.9	534-536 bp	1.0	79.6