Hotspot

atpI-atpH

Genome · 39066-39737 · 672 bp

Back to Torreya

Pi 0.00565

MarkerSeek score 56.50

Primer available yes

Label atpI-atpH

Feature Metadata

label_name: atpI-atpH
feature_type: igs
region: Genome
length_bp: 672
variable_sites: 7
pi: 0.00565
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-atpH_p1	TAGGTGAATCCATGGAGGGT	TCCAATAGAAGCGAGGCCTA	50.0	50.0	57.4	57.9	766-771 bp	1.0	76.2
atpI-atpH_p2	TAGGTGAATCCATGGAGGGT	CCTACGGATAATCCAGCAGC	50.0	55.0	57.4	57.9	750-755 bp	1.0	76.2
atpI-atpH_p3	TAGGTGAATCCATGGAGGGT	GCCTACGGATAATCCAGCAG	50.0	55.0	57.4	57.9	751-756 bp	1.0	76.2
atpI-atpH_p4	TAGGTGAATCCATGGAGGGT	AACGGAAGCAGCAGAAATCA	50.0	45.0	57.4	58.1	727-732 bp	1.0	76.1
atpI-atpH_p5	TAGGTGAATCCATGGAGGGT	ACGGAAGCAGCAGAAATCAA	50.0	45.0	57.4	58.1	726-731 bp	1.0	76.1