Hotspot

trnK-UUU-rps16

Genome · 3935-4708 · 774 bp

Back to Torreya

Pi 0.00762

MarkerSeek score 64.90

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: Genome
length_bp: 774
variable_sites: 13
pi: 0.00762
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AGTCGCACTTAAAAGCCGAG	GCTCAACCTACGGAAACTGT	50.0	50.0	58.6	57.8	897-910 bp	1.0	76.5
trnK-UUU-rps16_p2	AGTCGCACTTAAAAGCCGAG	GGAGCTCAACCTACGGAAAC	50.0	55.0	58.6	58.3	900-913 bp	1.0	75.6
trnK-UUU-rps16_p3	TAGTCGCACTTAAAAGCCGA	GCTCAACCTACGGAAACTGT	45.0	50.0	57.3	57.8	898-911 bp	1.0	75.2
trnK-UUU-rps16_p4	TCGCACTTAAAAGCCGAGTA	GCTCAACCTACGGAAACTGT	45.0	50.0	57.3	57.8	895-908 bp	1.0	75.2
trnK-UUU-rps16_p5	AGTCGCACTTAAAAGCCGAG	AGGAGCTCAACCTACGGAAA	50.0	50.0	58.6	58.4	901-914 bp	1.0	74.9