Hotspot

matK

LSC · 2182-3696 · 1515 bp

Back to Torminalis

Pi 0

MarkerSeek score 40.00

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: LSC
length_bp: 1515
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	CCATTTCTACGATCGCATAACC	TGAGTCTTACTTGTTCCGAGG	45.5	47.6	57.4	57.1	1657 bp	1.0	43.3
matK_p2	CCATTTCTACGATCGCATAACC	GAGTCTTACTTGTTCCGAGGT	45.5	47.6	57.4	57.1	1656 bp	1.0	43.3
matK_p3	TTTCCATTTCTACGATCGCA	TGAGTCTTACTTGTTCCGAGG	40.0	47.6	55.2	57.1	1660 bp	1.0	42.0
matK_p4	TTTCCATTTCTACGATCGCA	GAGTCTTACTTGTTCCGAGGT	40.0	47.6	55.2	57.1	1659 bp	1.0	42.0
matK_p5	CCATTTCTACGATCGCATAACC	AGTCTTACTTGTTCCGAGGT	45.5	45.0	57.4	55.8	1655 bp	1.0	41.3