Hotspot

trnH-GUG-psbA

LSC · 300-577 · 278 bp

Back to Torminalis

Pi 0
MarkerSeek score 52.80
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
278
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG GCGCTAACCTTGGTATGGAA 55.0 50.0 58.1 58.0 394-395 bp 1.0 78.9
trnH-GUG-psbA_p2 CAATCCACTGCCTTGATCCA GCGCTAACCTTGGTATGGAA 50.0 50.0 57.9 58.0 408-409 bp 1.0 78.9
trnH-GUG-psbA_p3 ATATTATGGGCGAACGACGG GCGCTAACCTTGGTATGGAA 50.0 50.0 57.9 58.0 456 bp 0.5 59.1
trnH-GUG-psbA_p4 ATATTATGGGCGAACGACGG CCTCTAGACCTAGCTGCTGT 50.0 55.0 57.9 57.9 406 bp 0.5 58.9
trnH-GUG-psbA_p5 ATATTATGGGCGAACGACGG TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.9 57.9 409 bp 0.5 58.8