Hotspot

ycf1

SSC · 123060-128627 · 5568 bp

Back to Torenia

Pi 0.0145

MarkerSeek score 45.40

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5568
variable_sites: 329
pi: 0.0145
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGGGTGATGGTATTCTGCCT	TGTTAGTTGGTTAATTGGTCACA	50.0	34.8	58.4	56.3	209 bp	1.0	46.9
ycf1_p2	ATGGGTGATGGTATTCTGCC	TGTTAGTTGGTTAATTGGTCACA	50.0	34.8	57.3	56.3	210 bp	1.0	46.9
ycf1_p3	TGGGTGATGGTATTCTGCCT	TTGTTAGTTGGTTAATTGGTCACA	50.0	33.3	58.4	57.0	210 bp	1.0	46.9
ycf1_p4	TGGGTGATGGTATTCTGCCT	TGTTAGTTGGTTAATTGGTCACAT	50.0	33.3	58.4	56.8	209 bp	1.0	46.9
ycf1_p5	ATGGGTGATGGTATTCTGCC	TTGTTAGTTGGTTAATTGGTCACA	50.0	33.3	57.3	57.0	211 bp	1.0	46.8