Hotspot

ndhF

SSC · 109686-111944 · 2259 bp

Back to Torenia

Pi 0.00994

MarkerSeek score 52.50

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2259
variable_sites: 102
pi: 0.00994
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	GAGCTACTGAGGAACAACGG	ACGCCGCTTTCCTAATTCAT	55.0	45.0	58.0	58.0	2850-2976 bp	1.0	88.1
ndhF_p2	TCGAACCCTCCTTCGTAACT	ACGCCGCTTTCCTAATTCAT	50.0	45.0	58.1	58.0	2775-2901 bp	1.0	87.6
ndhF_p3	CAATTCCCGTTCTCAACCCA	ACGCCGCTTTCCTAATTCAT	50.0	45.0	58.1	58.0	2809-2935 bp	1.0	87.4
ndhF_p4	CGTTCTCAACCCATGACCAA	ACGCCGCTTTCCTAATTCAT	50.0	45.0	58.1	58.0	2802-2928 bp	1.0	87.3
ndhF_p5	CTTCGTATTGGCTCCCTTCC	ACGCCGCTTTCCTAATTCAT	55.0	45.0	58.0	58.0	2804-2871 bp	0.916667	82.1