Hotspot

ndhA-ndhH

SSC · 121103-121103 · 1 bp

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Pi 0.303

MarkerSeek score 68.80

Primer available yes

Label ndhA-ndhH

Feature Metadata

label_name: ndhA-ndhH
feature_type: igs
region: SSC
length_bp: 1
variable_sites: 1
pi: 0.303
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA-ndhH_p1	GGGGCAAGCATCCATATGAT	CACCGGGTTTTATCAACTTGC	50.0	47.6	57.8	58.1	212 bp	0.916667	67.9
ndhA-ndhH_p2	AAAATAGGGGCAAGCATCCA	CACCGGGTTTTATCAACTTGC	45.0	47.6	57.5	58.1	218 bp	0.916667	65.3
ndhA-ndhH_p3	GGGGCAAGCATCCATATGAT	CCGGGTTTTATCAACTTGCA	50.0	45.0	57.8	56.6	210 bp	0.916667	65.3
ndhA-ndhH_p4	GGGGCAAGCATCCATATGAT	ACCGGGTTTTATCAACTTGC	50.0	45.0	57.8	56.6	211 bp	0.916667	65.3
ndhA-ndhH_p5	GGGGCAAGCATCCATATGAT	ACCGGGTTTTATCAACTTGCA	50.0	42.9	57.8	58.4	211 bp	0.916667	65.1