Hotspot

rpl22-rps19

LSC · 84777-84853 · 77 bp

Back to Torenia

Pi 0.0288
MarkerSeek score 65.20
Primer available yes
Label rpl22-rps19

Feature Metadata

label_name
rpl22-rps19
feature_type
igs
region
LSC
length_bp
77
variable_sites
9
pi
0.0288
unique_haplotype_count
10
primer_available
yes
alignment_reliability
0.97
markerseek_score
65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl22-rps19_p1 TCGACCTAAAGCGTATACTTCG TTCGAGGCCATTCAAAAAGC 45.5 45.0 57.8 57.3 160-180 bp 1.0 64.8
rpl22-rps19_p2 GACCTAAAGCGTATACTTCGGT TTCGAGGCCATTCAAAAAGC 45.5 45.0 57.7 57.3 158-178 bp 1.0 64.1
rpl22-rps19_p3 TCGACCTAAAGCGTATACTTCG GCGATAATAGGTCTCGTCGT 45.5 50.0 57.8 57.0 142-162 bp 1.0 63.9
rpl22-rps19_p4 TCGACCTAAAGCGTATACTTCG GAGGCCATTCAAAAAGCGAT 45.5 45.0 57.8 57.1 157-177 bp 1.0 63.2
rpl22-rps19_p5 ACCTAAAGCGTATACTTCGGT TTCGAGGCCATTCAAAAAGC 42.9 45.0 56.6 57.3 157-177 bp 1.0 63.1