Hotspot

psbE-petL

LSC · 65955-66881 · 927 bp

Back to Torenia

Pi 0.0169

MarkerSeek score 58.10

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 927
variable_sites: 63
pi: 0.0169
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 58.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	GGTTCAAGTCCTACAGAGCG	50.0	55.0	57.9	58.0	1543-1574 bp	1.0	85.7
psbE-petL_p2	ATGGAATTCCTTGTCGGCTC	GGTTCAAGTCCTACAGAGCG	50.0	55.0	58.0	58.0	1632-1663 bp	1.0	85.5
psbE-petL_p3	ATGGAATTCCTTGTCGGCTC	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	58.0	58.0	1685-1716 bp	1.0	85.2
psbE-petL_p4	TCGTTCGGGTTTGATTGTGT	GGTTCAAGTCCTACAGAGCG	45.0	55.0	58.0	58.0	1871-1902 bp	1.0	84.8
psbE-petL_p5	TCGTTCGGGTTTGATTGTGT	AGCGCTCTTAGTTCAGTTCG	45.0	50.0	58.0	58.0	1924-1955 bp	1.0	84.5