Hotspot

trnT-UGU-trnL-UAA

LSC · 47590-48253 · 664 bp

Back to Torenia

Pi 0.00847
MarkerSeek score 62.20
Primer available yes
Label trnT-UGU-trnL-UAA

Feature Metadata

label_name
trnT-UGU-trnL-UAA
feature_type
igs
region
LSC
length_bp
664
variable_sites
26
pi
0.00847
unique_haplotype_count
10
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnL-UAA_p1 GCGATGCTCTAACCTCTGAG CCGTAGCGTCTACCGATTTC 55.0 55.0 57.9 58.2 723-782 bp 1.0 81.0
trnT-UGU-trnL-UAA_p2 TAGGAAAAAGCCGGCTATCG CCGTAGCGTCTACCGATTTC 50.0 55.0 57.8 58.2 774-833 bp 1.0 80.0
trnT-UGU-trnL-UAA_p3 GGAATCGAACCGATGACCAT CCGTAGCGTCTACCGATTTC 50.0 55.0 57.8 58.2 755-814 bp 1.0 80.0
trnT-UGU-trnL-UAA_p4 GCGATGCTCTAACCTCTGAG CGTCTACCGATTTCGCCATA 55.0 50.0 57.9 57.6 717-776 bp 1.0 79.8
trnT-UGU-trnL-UAA_p5 GCGATGCTCTAACCTCTGAG GGTTTCCATACCAAGGCTCA 55.0 50.0 57.9 57.5 755-814 bp 1.0 78.9