Hotspot

psaA-ycf3

LSC · 42640-43366 · 727 bp

Back to Torenia

Pi 0.0135

MarkerSeek score 71.20

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 727
variable_sites: 40
pi: 0.0135
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	TGAGAAATGACCCGGTTTGG	ATCAAGCCGCTGAGTATTGG	50.0	50.0	58.1	58.0	924-1005 bp	1.0	85.7
psaA-ycf3_p2	TGAGAAATGACCCGGTTTGG	ATAGCGCTGACTCCTGGTAA	50.0	50.0	58.1	58.2	895-976 bp	1.0	84.5
psaA-ycf3_p3	TGAGAAATGACCCGGTTTGG	TAGCGCTGACTCCTGGTAAT	50.0	50.0	58.1	58.2	894-975 bp	1.0	84.5
psaA-ycf3_p4	TGAGAAATGACCCGGTTTGG	AGCCGCTGAGTATTGGAAAC	50.0	50.0	58.1	58.3	920-1001 bp	1.0	84.0
psaA-ycf3_p5	TGAGAAATGACCCGGTTTGG	CAAGCCGCTGAGTATTGGAA	50.0	50.0	58.1	58.3	922-1003 bp	1.0	84.0