Hotspot

atpF-atpH

LSC · 13606-13985 · 380 bp

Back to Torenia

Pi 0.0161

MarkerSeek score 62.40

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 380
variable_sites: 28
pi: 0.0161
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	TTAAATCCGAAACTCCCGGC	CCGAGGCAGAGGGAAAAATA	50.0	50.0	58.3	57.3	565-594 bp	1.0	76.6
atpF-atpH_p2	TTAAATCCGAAACTCCCGGC	GCAGAGGGAAAAATACGAGGT	50.0	47.6	58.3	58.0	560-589 bp	1.0	74.2
atpF-atpH_p3	TTAAATCCGAAACTCCCGGC	GGGTTGGTTGTAGCATTAGC	50.0	50.0	58.3	56.8	494-523 bp	1.0	73.1
atpF-atpH_p4	TTAAATCCGAAACTCCCGGC	GAGGCAGAGGGAAAAATACGA	50.0	47.6	58.3	57.7	563-592 bp	1.0	72.2
atpF-atpH_p5	TTAAATCCGAAACTCCCGGC	AGGCAGAGGGAAAAATACGAG	50.0	47.6	58.3	57.7	562-591 bp	1.0	72.1