Hotspot

trnG-CGA-trnR-UCU

LSC · 10337-10580 · 244 bp

Back to Torenia

Pi 0.027

MarkerSeek score 67.40

Primer available yes

Label trnG-CGA-trnR-UCU

Feature Metadata

label_name: trnG-CGA-trnR-UCU
feature_type: igs
region: LSC
length_bp: 244
variable_sites: 21
pi: 0.027
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 67.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-CGA-trnR-UCU_p1	ACCCTTAGCCTTCCAAGCTA	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.0	56.9	310-403 bp	1.0	72.6
trnG-CGA-trnR-UCU_p2	AGCCTTCCAAGCTAACGATG	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.0	56.9	304-397 bp	1.0	72.5
trnG-CGA-trnR-UCU_p3	ACCCTTAGCCTTCCAAGCTA	AGGTTTAGAAGACCTCTGTCCT	50.0	45.5	58.0	57.9	316-409 bp	1.0	71.9
trnG-CGA-trnR-UCU_p4	AGCCTTCCAAGCTAACGATG	AGGTTTAGAAGACCTCTGTCCT	50.0	45.5	58.0	57.9	310-403 bp	1.0	71.8
trnG-CGA-trnR-UCU_p5	CAAAACGCTGAATCGACGTC	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.1	56.9	337-430 bp	1.0	71.3