Hotspot

rpl32-trnL-UAG

Genome · 113409-114334 · 926 bp

Back to Tordylium

Pi 0.0424
MarkerSeek score 80.50
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
Genome
length_bp
926
variable_sites
55
pi
0.0424
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.98
markerseek_score
80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GCAGCCTTAAAAGCGTTGTC AGCGTGTCTACCGATTTCAC 50.0 50.0 58.3 58.0 1017-1066 bp 1.0 85.0
rpl32-trnL-UAG_p2 GCAGCCTTAAAAGCGTTGTC CAGCGTGTCTACCGATTTCA 50.0 50.0 58.3 58.0 1018-1067 bp 1.0 84.9
rpl32-trnL-UAG_p3 GCAGCCTTAAAAGCGTTGTC CTCGCACTGCTTCCTAAGAG 50.0 55.0 58.3 58.1 1038-1087 bp 1.0 84.2
rpl32-trnL-UAG_p4 GCAGCCTTAAAAGCGTTGTC CTCTCGCACTGCTTCCTAAG 50.0 55.0 58.3 58.1 1040-1089 bp 1.0 84.2
rpl32-trnL-UAG_p5 GCAGCCTTAAAAGCGTTGTC TCCTAAGAGCAGCGTGTCTA 50.0 50.0 58.3 57.9 1027-1076 bp 1.0 84.0