Hotspot

ycf1-ndhF

Genome · 110020-110055 · 36 bp

Back to Tordylium

Pi 0.0762

MarkerSeek score 77.50

Primer available yes

Label ycf1-ndhF

Feature Metadata

label_name: ycf1-ndhF
feature_type: igs
region: Genome
length_bp: 36
variable_sites: 4
pi: 0.0762
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 77.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-ndhF_p1	CCCTGCCCACACTAGAAAAA	GTTGGATCCCTCGGAATTCC	50.0	55.0	57.7	58.0	1226-1296 bp	1.0	82.1
ycf1-ndhF_p2	TACGTATCCGCCTAGCTTGT	GTTGGATCCCTCGGAATTCC	50.0	55.0	58.3	58.0	1279-1349 bp	1.0	81.7
ycf1-ndhF_p3	CCCTGCCCACACTAGAAAAA	TGGATCCCTCGGAATTCCTT	50.0	50.0	57.7	58.1	1224-1294 bp	1.0	81.5
ycf1-ndhF_p4	CCCTGCCCACACTAGAAAAA	TTGGATCCCTCGGAATTCCT	50.0	50.0	57.7	58.1	1225-1295 bp	1.0	81.5
ycf1-ndhF_p5	CCCTGCCCACACTAGAAAAA	GGGGAATAAGAGGATTGGCG	50.0	55.0	57.7	58.1	911 bp	0.333333	50.2