Pi
0.0462
MarkerSeek score
77.00
Primer available
yes
Label
trnT-UGU-trnL-UAA
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnT-UGU-trnL-UAA_p1 | GCGATGCTCTAACCTCTGAG | CTTTTTCACCTTCTGGGCCT | 55.0 | 50.0 | 57.9 | 58.0 | 967-1002 bp | 1.0 | 85.5 |
| trnT-UGU-trnL-UAA_p2 | AAAACCTGACCTGTTGACCC | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 57.9 | 58.0 | 1471-1569 bp | 1.0 | 85.0 |
| trnT-UGU-trnL-UAA_p3 | AAAACCTGACCTGTTGACCC | CTTTTTCACCTTCTGGGCCT | 50.0 | 50.0 | 57.9 | 58.0 | 1625-1723 bp | 1.0 | 84.6 |
| trnT-UGU-trnL-UAA_p4 | TAGTCGGAGCCATACCCAAT | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 57.9 | 58.0 | 1533-1631 bp | 1.0 | 84.5 |
| trnT-UGU-trnL-UAA_p5 | TAGTCGGAGCCATACCCAAT | CTTTTTCACCTTCTGGGCCT | 50.0 | 50.0 | 57.9 | 58.0 | 1687-1785 bp | 1.0 | 84.2 |