Hotspot

trnY-GUA-trnE-UUC

Genome · 29846-29920 · 75 bp

Back to Tordylium

Pi 0.0711

MarkerSeek score 76.80

Primer available yes

Label trnY-GUA-trnE-UUC

Feature Metadata

label_name: trnY-GUA-trnE-UUC
feature_type: igs
region: Genome
length_bp: 75
variable_sites: 8
pi: 0.0711
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnY-GUA-trnE-UUC_p1	CCAGCGTAGACATATTGCCA	TCTCTTTCAAGGAGGCAACG	50.0	50.0	57.8	57.8	183 bp	1.0	80.2
trnY-GUA-trnE-UUC_p2	ACCAGCGTAGACATATTGCC	TCTCTTTCAAGGAGGCAACG	50.0	50.0	57.8	57.8	184 bp	1.0	80.2
trnY-GUA-trnE-UUC_p3	CGAATTTACAGTCCGTCCCC	TCTCTTTCAAGGAGGCAACG	55.0	50.0	58.4	57.8	162 bp	1.0	79.8
trnY-GUA-trnE-UUC_p4	ACGAATTTACAGTCCGTCCC	TCTCTTTCAAGGAGGCAACG	50.0	50.0	57.6	57.8	163 bp	1.0	79.6
trnY-GUA-trnE-UUC_p5	GCCAACGAATTTACAGTCCG	TCTCTTTCAAGGAGGCAACG	50.0	50.0	57.5	57.8	167 bp	1.0	78.3