Hotspot

psbA-trnK-UUU

Genome · 1331-1530 · 200 bp

Back to Tordylium

Pi 0.0533

MarkerSeek score 82.70

Primer available yes

Label psbA-trnK-UUU

Feature Metadata

label_name: psbA-trnK-UUU
feature_type: igs
region: Genome
length_bp: 200
variable_sites: 15
pi: 0.0533
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-UUU_p1	TAAAGACGGTTTTCGGTGCT	GGGGAGGGGTGTTTACCTAT	45.0	55.0	57.8	58.1	364-369 bp	1.0	82.3
psbA-trnK-UUU_p2	TAAAGACGGTTTTCGGTGCT	CTAGTTCCGGGTTCGAATCC	45.0	55.0	57.8	57.8	315-320 bp	1.0	82.1
psbA-trnK-UUU_p3	GTAAAGACGGTTTTCGGTGC	GGGGAGGGGTGTTTACCTAT	50.0	55.0	57.7	58.1	365-370 bp	1.0	81.8
psbA-trnK-UUU_p4	TAAAGACGGTTTTCGGTGCT	GAAAGCCGTGTGCAATGAAA	45.0	45.0	57.8	57.9	400-405 bp	1.0	81.6
psbA-trnK-UUU_p5	GTAAAGACGGTTTTCGGTGC	GAAAGCCGTGTGCAATGAAA	50.0	45.0	57.7	57.9	401-406 bp	1.0	81.1