Hotspot

rrn23

IRb · 108354-111163 · 2810 bp

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Pi 0.00173
MarkerSeek score 41.50
Primer available yes
Label rrn23

Feature Metadata

label_name
rrn23
feature_type
rRNA
region
IRb
length_bp
2810
variable_sites
17
pi
0.00173
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
41.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rrn23_p1 TCCTTCCATTTAGCAGCACC AAACTTCTGTCGCTCCATCC 50.0 50.0 57.9 57.9 2954 bp 1.0 78.6
rrn23_p2 TCCTTCCATTTAGCAGCACC GCCGTGACCTTCTCTTGAAT 50.0 50.0 57.9 57.9 2979 bp 1.0 78.6
rrn23_p3 CCTTCCATTTAGCAGCACCT AAACTTCTGTCGCTCCATCC 50.0 50.0 57.9 57.9 2953 bp 1.0 78.6
rrn23_p4 CCTTCCATTTAGCAGCACCT GCCGTGACCTTCTCTTGAAT 50.0 50.0 57.9 57.9 2978 bp 1.0 78.6
rrn23_p5 CGGCAGTTCGATCTATGGTT AAACTTCTGTCGCTCCATCC 50.0 50.0 57.8 57.9 2998 bp 1.0 77.8