Hotspot

trnP-GGG-psaJ

LSC · 70221-70583 · 363 bp

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Pi 0.00367
MarkerSeek score 29.90
Primer available yes
Label trnP-GGG-psaJ

Feature Metadata

label_name
trnP-GGG-psaJ
feature_type
igs
region
LSC
length_bp
363
variable_sites
4
pi
0.00367
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.98
markerseek_score
29.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnP-GGG-psaJ_p1 CTACCAAGCTGCGCTACATC GGTGCCACGGAAAGATATGT 55.0 50.0 58.8 58.0 411-420 bp 1.0 75.9
trnP-GGG-psaJ_p2 CTACCAAGCTGCGCTACATC AACCTGCTAAAGACCCGAAC 55.0 50.0 58.8 57.8 449-458 bp 1.0 74.6
trnP-GGG-psaJ_p3 CTACCAAGCTGCGCTACATC AGTACTTAGTACCGGTGCCA 55.0 50.0 58.8 57.8 424-433 bp 1.0 74.4
trnP-GGG-psaJ_p4 CTACCAAGCTGCGCTACATC GTACTTAGTACCGGTGCCAC 55.0 55.0 58.8 57.7 423-432 bp 1.0 73.9
trnP-GGG-psaJ_p5 CTACCAAGCTGCGCTACATC GTCAACGCATCCGGGAATAA 55.0 50.0 58.8 58.3 489-498 bp 1.0 73.1