Hotspot

psbE-petL

LSC · 68101-69380 · 1280 bp

Back to Toona

Pi 0.00595
MarkerSeek score 38.50
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1280
variable_sites
26
pi
0.00595
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
38.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 ATGGAATTCCTTGTCGGCTC AGCGCTCTTAGTTCAGTTCG 50.0 50.0 58.0 58.0 2065-2132 bp 1.0 81.5
psbE-petL_p2 CAAGTGGTCGTGCTAGAGAC AGCGCTCTTAGTTCAGTTCG 55.0 50.0 58.0 58.0 2508-2575 bp 1.0 81.4
psbE-petL_p3 TCGTTCGGGTTTGATTGTGT AGCGCTCTTAGTTCAGTTCG 45.0 50.0 58.0 58.0 2298-2365 bp 1.0 81.3
psbE-petL_p4 GGTAGGTACAGCTAGTCCGT AGCGCTCTTAGTTCAGTTCG 55.0 50.0 58.0 58.0 2205-2272 bp 1.0 81.3
psbE-petL_p5 CAAGTGGTCGTGCTAGAGAC AGAACGTGGGTCTCCAAAAC 55.0 50.0 58.0 58.0 2486-2553 bp 1.0 81.2