Hotspot

ycf4-cemA

LSC · 63440-64381 · 942 bp

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Pi 0.00627
MarkerSeek score 41.50
Primer available yes
Label ycf4-cemA

Feature Metadata

label_name
ycf4-cemA
feature_type
igs
region
LSC
length_bp
942
variable_sites
20
pi
0.00627
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
41.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf4-cemA_p1 TTGGCCTATTTCTTGCGTGT AGATCCACCAGGGCAAAAAG 45.0 50.0 58.1 58.1 1049-1061 bp 1.0 81.2
ycf4-cemA_p2 TTGGCCTATTTCTTGCGTGT GAGATCCACCAGGGCAAAAA 45.0 50.0 58.1 58.1 1050-1062 bp 1.0 81.1
ycf4-cemA_p3 TTGGCCTATTTCTTGCGTGT GATCCACCAGGGCAAAAAGA 45.0 50.0 58.1 58.1 1048-1060 bp 1.0 81.1
ycf4-cemA_p4 TTGGCCTATTTCTTGCGTGT TCGAAGGGGAATGGATACGT 45.0 50.0 58.1 58.2 1003-1015 bp 1.0 80.1
ycf4-cemA_p5 TTGGCCTATTTCTTGCGTGT CCACCAGGGCAAAAAGACTA 45.0 50.0 58.1 57.7 1045-1057 bp 1.0 79.5