Hotspot

psbC-trnS-UGA

LSC · 37452-37688 · 237 bp

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Pi 0.0207
MarkerSeek score 40.70
Primer available yes
Label psbC-trnS-UGA

Feature Metadata

label_name
psbC-trnS-UGA
feature_type
igs
region
LSC
length_bp
237
variable_sites
9
pi
0.0207
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbC-trnS-UGA_p1 TCCATGACTCCTCTTAACTGA GGTTGATAGCTCCGGTCTTG 42.9 55.0 55.4 58.1 335 bp 1.0 53.4
psbC-trnS-UGA_p2 GTTCTTTCCATGACTCCTCT GGTTGATAGCTCCGGTCTTG 45.0 55.0 54.4 58.1 341 bp 1.0 52.9
psbC-trnS-UGA_p3 TCCATGACTCCTCTTAACTGA GATGGCCGAGTGGTTGATAG 42.9 55.0 55.4 58.1 346 bp 1.0 52.7
psbC-trnS-UGA_p4 TCCATGACTCCTCTTAACTGA GGGTTCGAATCCCTCTCTCT 42.9 55.0 55.4 58.2 281 bp 1.0 52.6
psbC-trnS-UGA_p5 GTTCTTTCCATGACTCCTCT GATGGCCGAGTGGTTGATAG 45.0 55.0 54.4 58.1 352 bp 1.0 52.2