Hotspot

rps15-ycf1

SSC · 126041-126411 · 371 bp

Back to Tongoloa

Pi 0.0383

MarkerSeek score 68.40

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 371
variable_sites: 65
pi: 0.0383
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	GACCCCTTGTTGTCTTCGTT	GAGCCCATTCGTTTGTCTGT	50.0	50.0	58.0	58.5	952-1014 bp	1.0	87.6
rps15-ycf1_p2	GGTGCTGAGGACCCATATTG	GAGCCCATTCGTTTGTCTGT	55.0	50.0	58.0	58.5	1312-1374 bp	1.0	86.2
rps15-ycf1_p3	GGTCATTTCGTGTAGCTGGT	GAGCCCATTCGTTTGTCTGT	50.0	50.0	57.9	58.5	1282-1344 bp	1.0	85.9
rps15-ycf1_p4	ATGAGTCGAAGAACACCGTG	GAGCCCATTCGTTTGTCTGT	50.0	50.0	57.9	58.5	1340-1402 bp	1.0	85.8
rps15-ycf1_p5	CCTAACTGCTCTGGTGCATT	GAGCCCATTCGTTTGTCTGT	50.0	50.0	57.9	58.5	1523-1585 bp	1.0	84.4