Hotspot

rpl32-trnL-UAG

SSC · 115942-116667 · 726 bp

Back to Tongoloa

Pi 0.0295

MarkerSeek score 79.40

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 726
variable_sites: 74
pi: 0.0295
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TACTGGGCAGCCTTAAAAGC	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.2	58.0	594-851 bp	1.0	90.5
rpl32-trnL-UAG_p2	TACTGGGCAGCCTTAAAAGC	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.2	58.0	595-852 bp	1.0	90.4
rpl32-trnL-UAG_p3	TACTGGGCAGCCTTAAAAGC	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.2	57.9	604-861 bp	1.0	89.4
rpl32-trnL-UAG_p4	GCAGCCTTAAAAGCGTTGTC	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.3	58.0	588-845 bp	1.0	89.3
rpl32-trnL-UAG_p5	GCAGCCTTAAAAGCGTTGTC	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.3	58.0	589-846 bp	1.0	89.3