Hotspot

ndhF-rpl32

SSC · 114585-115776 · 1192 bp

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Pi 0.0231

MarkerSeek score 74.30

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1192
variable_sites: 116
pi: 0.0231
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 74.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AGGAAAGGTATTATCCACGCA	TAAGGCTGCCCAGTATCCTT	42.9	50.0	56.8	58.1	1149-1401 bp	1.0	72.8
ndhF-rpl32_p2	AGGAAAGGTATTATCCACGCA	TTAAGGCTGCCCAGTATCCT	42.9	50.0	56.8	58.1	1150-1402 bp	1.0	72.8
ndhF-rpl32_p3	AGGAAAGGTATTATCCACGCA	GCTTTTAAGGCTGCCCAGTA	42.9	50.0	56.8	58.2	1154-1406 bp	1.0	72.3
ndhF-rpl32_p4	AGGAAAGGTATTATCCACGCA	GACAACGCTTTTAAGGCTGC	42.9	50.0	56.8	58.3	1160-1412 bp	1.0	71.1
ndhF-rpl32_p5	AGGAAAGGTATTATCCACGCA	TGCCCAGTATCCTTTCCCTT	42.9	50.0	56.8	58.3	1143-1395 bp	1.0	71.1