Hotspot

rpl33-rps18

LSC · 69646-69893 · 248 bp

Back to Tongoloa

Pi 0.067

MarkerSeek score 76.90

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 248
variable_sites: 46
pi: 0.067
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	ACAAACATACGATTCATGGGGA	TCAATTCGATCCCCCGATTG	40.9	50.0	57.8	57.7	328-404 bp	1.0	72.1
rpl33-rps18_p2	ACATACGATTCATGGGGAGA	TCAATTCGATCCCCCGATTG	45.0	50.0	55.7	57.7	324-400 bp	1.0	71.6
rpl33-rps18_p3	AACATACGATTCATGGGGAGA	TCAATTCGATCCCCCGATTG	42.9	50.0	56.5	57.7	325-401 bp	1.0	70.0
rpl33-rps18_p4	ACAAACATACGATTCATGGGGA	ATTCGATCCCCCGATTGGAT	40.9	50.0	57.8	58.6	325-401 bp	1.0	69.1
rpl33-rps18_p5	ACATACGATTCATGGGGAGA	ATTCGATCCCCCGATTGGAT	45.0	50.0	55.7	58.6	321-397 bp	1.0	68.6