Hotspot

trnW-CCA-trnP-UGG

LSC · 68222-68398 · 177 bp

Back to Tongoloa

Pi 0.0543

MarkerSeek score 80.40

Primer available yes

Label trnW-CCA-trnP-UGG

Feature Metadata

label_name: trnW-CCA-trnP-UGG
feature_type: igs
region: LSC
length_bp: 177
variable_sites: 31
pi: 0.0543
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnW-CCA-trnP-UGG_p1	GCGTTCTACCGAACTGAACT	CTTGGTAGCGCGTTTGTTTT	50.0	45.0	57.9	57.9	217-281 bp	1.0	90.6
trnW-CCA-trnP-UGG_p2	ACCTACGACATCGGGTTTTG	CTTGGTAGCGCGTTTGTTTT	50.0	45.0	57.9	57.9	244-308 bp	1.0	90.4
trnW-CCA-trnP-UGG_p3	GCGTTCTACCGAACTGAACT	TTGGTAGCGCGTTTGTTTTG	50.0	45.0	57.9	58.2	216-280 bp	1.0	90.1
trnW-CCA-trnP-UGG_p4	GCGTTCTACCGAACTGAACT	CGCGTTTGTTTTGGGTACAA	50.0	45.0	57.9	57.8	209-273 bp	1.0	90.0
trnW-CCA-trnP-UGG_p5	ACCTACGACATCGGGTTTTG	TTGGTAGCGCGTTTGTTTTG	50.0	45.0	57.9	58.2	243-307 bp	1.0	89.9