Hotspot

petA-psbJ

LSC · 64686-65656 · 971 bp

Back to Tongoloa

Pi 0.0237
MarkerSeek score 78.20
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
971
variable_sites
92
pi
0.0237
unique_haplotype_count
14
primer_available
yes
alignment_reliability
0.97
markerseek_score
78.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 GGCATCTGTTATTTTGGCACA ATAGGTGCTGTAGCGGGTAT 42.9 50.0 57.4 58.0 1096-1171 bp 0.928571 74.7
petA-psbJ_p2 TGGCATCTGTTATTTTGGCAC ATAGGTGCTGTAGCGGGTAT 42.9 50.0 57.4 58.0 1097-1172 bp 0.928571 74.7
petA-psbJ_p3 GGCATCTGTTATTTTGGCACA AATAGGTGCTGTAGCGGGTA 42.9 50.0 57.4 58.2 1097-1172 bp 0.928571 73.1
petA-psbJ_p4 GGCATCTGTTATTTTGGCACA TAATAGGTGCTGTAGCGGGT 42.9 50.0 57.4 58.2 1098-1173 bp 0.928571 73.1
petA-psbJ_p5 GGCATCTGTTATTTTGGCACA TAGGTGCTGTAGCGGGTATT 42.9 50.0 57.4 58.2 1095-1170 bp 0.928571 73.1