Hotspot

ndhC-trnV-UAC

LSC · 52415-53160 · 746 bp

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Pi 0.0146

MarkerSeek score 74.50

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 746
variable_sites: 48
pi: 0.0146
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GGGGCTAAAACTCCGGAAAT	CCGAGAAAGTCTACGGTTCG	50.0	55.0	57.9	58.1	873-900 bp	1.0	84.8
ndhC-trnV-UAC_p2	GGGGCTAAAACTCCGGAAAT	ACGCATGTTGGGTCTTTGAA	50.0	45.0	57.9	58.3	936-963 bp	1.0	82.9
ndhC-trnV-UAC_p3	TCAGGCCCTTTGTTAATCGG	CCGAGAAAGTCTACGGTTCG	50.0	55.0	57.9	58.1	892-918 bp	0.928571	81.1
ndhC-trnV-UAC_p4	TCAGGCCCTTTGTTAATCGG	AACGCATGTTGGGTCTTTGA	50.0	45.0	57.9	58.3	956-982 bp	0.928571	79.2
ndhC-trnV-UAC_p5	TCAGGCCCTTTGTTAATCGG	ACGCATGTTGGGTCTTTGAA	50.0	45.0	57.9	58.3	955-981 bp	0.928571	79.2