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psbZ-trnG-GCC

LSC · 37738-38031 · 294 bp

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Pi 0.0169

MarkerSeek score 68.80

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 294
variable_sites: 20
pi: 0.0169
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	TTGCTTCTCCTGATGGTTGG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.8	57.8	442-452 bp	1.0	84.9
psbZ-trnG-GCC_p2	GCTTCTCCTGATGGTTGGTC	CGTCTTCTCCTTGGCAAAGA	55.0	50.0	58.3	57.8	440-450 bp	1.0	84.5
psbZ-trnG-GCC_p3	TTGCTTCTCCTGATGGTTGG	TACCATTCGACCATATCCGC	50.0	50.0	57.8	57.3	415-425 bp	1.0	80.6
psbZ-trnG-GCC_p4	GCTTCTCCTGATGGTTGGTC	TACCATTCGACCATATCCGC	55.0	50.0	58.3	57.3	413-423 bp	1.0	80.3
psbZ-trnG-GCC_p5	CTCCTGATGGTTGGTCAAGT	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.1	57.8	436-446 bp	1.0	79.7