Hotspot

trnS-UGA-psbZ

LSC · 37202-37548 · 347 bp

Back to Tongoloa

Pi 0.022

MarkerSeek score 70.90

Primer available yes

Label trnS-UGA-psbZ

Feature Metadata

label_name: trnS-UGA-psbZ
feature_type: igs
region: LSC
length_bp: 347
variable_sites: 27
pi: 0.022
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 70.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-psbZ_p1	CAACCACTCAGCCATCTCTC	CCAACCATCAGGAGAAGCAA	55.0	50.0	58.0	57.8	464-499 bp	1.0	83.8
trnS-UGA-psbZ_p2	GAGCTATCAACCACTCAGCC	CCAACCATCAGGAGAAGCAA	55.0	50.0	58.1	57.8	471-506 bp	1.0	83.4
trnS-UGA-psbZ_p3	GGAGCTATCAACCACTCAGC	CCAACCATCAGGAGAAGCAA	55.0	50.0	58.1	57.8	472-507 bp	1.0	83.4
trnS-UGA-psbZ_p4	CAAGACCGGAGCTATCAACC	CCAACCATCAGGAGAAGCAA	55.0	50.0	58.1	57.8	479-514 bp	1.0	83.3
trnS-UGA-psbZ_p5	CAACCACTCAGCCATCTCTC	GACCAACCATCAGGAGAAGC	55.0	55.0	58.0	58.3	466-501 bp	1.0	83.3