Hotspot

rps2-rpoC2

LSC · 17261-17498 · 238 bp

Back to Tongoloa

Pi 0.0258

MarkerSeek score 71.30

Primer available yes

Label rps2-rpoC2

Feature Metadata

label_name: rps2-rpoC2
feature_type: igs
region: LSC
length_bp: 238
variable_sites: 22
pi: 0.0258
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 71.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2-rpoC2_p1	AATGAACTCCGGCCTTCATC	TTTGAGGGGGAAATGAGAGA	50.0	45.0	58.0	55.7	393-409 bp	1.0	70.6
rps2-rpoC2_p2	CCGGCCTTCATCATCTCTTC	TTTGAGGGGGAAATGAGAGA	55.0	45.0	58.1	55.7	385-401 bp	1.0	70.1
rps2-rpoC2_p3	CGGCCTTCATCATCTCTTCC	TTTGAGGGGGAAATGAGAGA	55.0	45.0	58.1	55.7	384-400 bp	1.0	70.1
rps2-rpoC2_p4	AATGAACTCCGGCCTTCATC	TTGAGGGGGAAATGAGAGAT	50.0	45.0	58.0	55.5	392-408 bp	1.0	68.7
rps2-rpoC2_p5	AAAATGAACTCCGGCCTTCA	TTTGAGGGGGAAATGAGAGA	45.0	45.0	57.7	55.7	395-411 bp	1.0	68.7