Hotspot

infA

LSC · 82280-82513 · 234 bp

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Pi 0.0057

MarkerSeek score 30.30

Primer available yes

Label infA

Feature Metadata

label_name: infA
feature_type: gene
region: LSC
length_bp: 234
variable_sites: 2
pi: 0.0057
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 30.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infA_p1	CTGCATCTCTTCCAAGACCC	GCTCGGGAATTGAGACAGTT	55.0	50.0	58.0	57.9	1634-1636 bp	1.0	82.4
infA_p2	CAGCAAATGGTGTCCCTCTT	GCTCGGGAATTGAGACAGTT	50.0	50.0	58.1	57.9	1535-1537 bp	1.0	82.1
infA_p3	TGCTGCATCTCTTCCAAGAC	GCTCGGGAATTGAGACAGTT	50.0	50.0	57.9	57.9	1636-1638 bp	1.0	81.9
infA_p4	GCTCGTAATGCTGCATCTCT	GCTCGGGAATTGAGACAGTT	50.0	50.0	58.1	57.9	1644-1646 bp	1.0	81.6
infA_p5	ACCAATACGTCCATTCCTGC	GCTCGGGAATTGAGACAGTT	50.0	50.0	58.0	57.9	1372-1374 bp	0.666667	65.6