Hotspot

ccsA-ndhD

SSC · 118163-118351 · 189 bp

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Pi 0.0741

MarkerSeek score 79.50

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 189
variable_sites: 21
pi: 0.0741
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TTTTTACCCAAGGCTTCGCT	TTCTCGATGGCTTCTCTTGC	45.0	50.0	58.0	58.0	1191-1199 bp	1.0	82.8
ccsA-ndhD_p2	TTTTTACCCAAGGCTTCGCT	CTCGATGGCTTCTCTTGCAT	45.0	50.0	58.0	58.0	1189-1197 bp	1.0	82.5
ccsA-ndhD_p3	TTTTTACCCAAGGCTTCGCT	CAACTTCTTTCGGTCAACGC	45.0	50.0	58.0	58.0	1454-1462 bp	1.0	82.3
ccsA-ndhD_p4	TTTTTACCCAAGGCTTCGCT	ATCGCCTACTCCTCTGTCTC	45.0	55.0	58.0	58.0	1419-1427 bp	1.0	82.3
ccsA-ndhD_p5	TTTTTACCCAAGGCTTCGCT	CCATGGTTGGTGATAGTGGG	45.0	55.0	58.0	57.9	1500-1508 bp	1.0	81.9