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petA-psbJ

LSC · 65428-66415 · 988 bp

Back to Tofieldia

Pi 0.0135

MarkerSeek score 66.20

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 988
variable_sites: 20
pi: 0.0135
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ACGTGTCCAAGGTCTTTTGT	TCTGGATTGGGCTCATCTCT	45.0	50.0	57.6	57.8	941-1128 bp	1.0	80.7
petA-psbJ_p2	ACGTGTCCAAGGTCTTTTGT	CTGGATTGGGCTCATCTCTG	45.0	55.0	57.6	57.7	940-1127 bp	1.0	80.1
petA-psbJ_p3	ACGTGTCCAAGGTCTTTTGT	TGGATTGGGCTCATCTCTGT	45.0	50.0	57.6	58.4	939-1126 bp	1.0	78.9
petA-psbJ_p4	TCCATTACGTGTCCAAGGTC	TCTGGATTGGGCTCATCTCT	50.0	50.0	57.2	57.8	947-1134 bp	1.0	78.0
petA-psbJ_p5	CCATTACGTGTCCAAGGTCT	TCTGGATTGGGCTCATCTCT	50.0	50.0	57.2	57.8	946-1133 bp	1.0	77.9