Hotspot

pafI-trnS-GGA

LSC · 45965-46242 · 278 bp

Back to Tofieldia

Pi 0.0242
MarkerSeek score 61.80
Primer available yes
Label pafI-trnS-GGA

Feature Metadata

label_name
pafI-trnS-GGA
feature_type
igs
region
LSC
length_bp
278
variable_sites
10
pi
0.0242
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
pafI-trnS-GGA_p1 TGGCTACAATTGAAGAGGTCT TTCCAATGCTACGCCTTGAA 42.9 45.0 56.6 57.8 359-374 bp 1.0 65.8
pafI-trnS-GGA_p2 TGGCTACAATTGAAGAGGTCTT TTCCAATGCTACGCCTTGAA 40.9 45.0 57.4 57.8 359-374 bp 1.0 63.5
pafI-trnS-GGA_p3 TTGGCTACAATTGAAGAGGTCT TTCCAATGCTACGCCTTGAA 40.9 45.0 57.4 57.8 360-375 bp 1.0 63.5
pafI-trnS-GGA_p4 TGGCTACAATTGAAGAGGTCT AGTTCCAATGCTACGCCTTG 42.9 50.0 56.6 58.5 361-376 bp 1.0 62.9
pafI-trnS-GGA_p5 TGGCTACAATTGAAGAGGTCT CAGTTCCAATGCTACGCCTT 42.9 50.0 56.6 58.5 362-377 bp 1.0 62.9