Hotspot

psbZ-trnG-GCC

LSC · 37428-38009 · 582 bp

Back to Tofieldia

Pi 0.0235
MarkerSeek score 63.60
Primer available yes
Label psbZ-trnG-GCC

Feature Metadata

label_name
psbZ-trnG-GCC
feature_type
igs
region
LSC
length_bp
582
variable_sites
18
pi
0.0235
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-GCC_p1 CTGGTAGCTATCCTTAATTCTCTCA AATCGAACCCGCATCTTCTC 40.0 50.0 57.5 58.1 499-710 bp 1.0 46.3
psbZ-trnG-GCC_p2 CTGGTAGCTATCCTTAATTCTCTCA GAATCGAACCCGCATCTTCT 40.0 50.0 57.5 58.1 500-711 bp 1.0 46.3
psbZ-trnG-GCC_p3 TGGTAGCTATCCTTAATTCTCTCA AATCGAACCCGCATCTTCTC 37.5 50.0 56.4 58.1 498-709 bp 1.0 46.3
psbZ-trnG-GCC_p4 TGGTAGCTATCCTTAATTCTCTCA GAATCGAACCCGCATCTTCT 37.5 50.0 56.4 58.1 499-710 bp 1.0 46.3
psbZ-trnG-GCC_p5 CTGGTAGCTATCCTTAATTCTCTCA TACTTCACCATGGGCGGATA 40.0 50.0 57.5 58.2 524-735 bp 1.0 46.2