Hotspot

psbI-trnS-GCU

LSC · 8504-8745 · 242 bp

Back to Tofieldia

Pi 0.0565

MarkerSeek score 75.60

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 242
variable_sites: 16
pi: 0.0565
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 75.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	GTAATCCTGGACGCGAAGAA	TAAAGCGGCGGATTGCTAAT	50.0	45.0	57.7	58.0	227-406 bp	1.0	85.4
psbI-trnS-GCU_p2	GTAATCCTGGACGCGAAGAA	GGGTTCGAATCCCTCTCTCT	50.0	55.0	57.7	58.2	182-361 bp	1.0	84.6
psbI-trnS-GCU_p3	GTAATCCTGGACGCGAAGAA	ATTGGGAGAGATGGCTGAGT	50.0	50.0	57.7	58.1	251-430 bp	1.0	84.5
psbI-trnS-GCU_p4	GTAATCCTGGACGCGAAGAA	TTCGAATCCCTCTCTCTCCG	50.0	55.0	57.7	58.3	179-358 bp	1.0	83.8
psbI-trnS-GCU_p5	GTAATCCTGGACGCGAAGAA	ATGGCTGAGTGGACTAAAGC	50.0	50.0	57.7	57.6	241-420 bp	1.0	82.2