Hotspot

ndhF

SSC · 110603-112822 · 2220 bp

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Pi 0.0587

MarkerSeek score 58.00

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2220
variable_sites: 36
pi: 0.0587
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.25
markerseek_score: 58.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ACAGGAACAAGAGGGATCCA	AGATCAGAATTGGTGAATCGGA	50.0	40.9	58.0	57.2	2441 bp	0.333333	30.9
ndhF_p2	AAGAGGGATCCACCGAAGAA	AGATCAGAATTGGTGAATCGGA	50.0	40.9	58.0	57.2	2433 bp	0.333333	30.9
ndhF_p3	AGAGGGATCCACCGAAGAAA	AGATCAGAATTGGTGAATCGGA	50.0	40.9	58.0	57.2	2432 bp	0.333333	30.9
ndhF_p4	ACAGGAACAAGAGGGATCCA	CAGAATTGGTGAATCGGAAACA	50.0	40.9	58.0	57.2	2437 bp	0.333333	30.9
ndhF_p5	AGAGGGATCCACCGAAGAAA	CAGAATTGGTGAATCGGAAACA	50.0	40.9	58.0	57.2	2428 bp	0.333333	30.8