Hotspot

ndhE

SSC · 118367-118687 · 321 bp

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Pi NA

MarkerSeek score 23.70

Primer available yes

Label ndhE

Feature Metadata

label_name: ndhE
feature_type: gene
region: SSC
length_bp: 321
variable_sites: 0
pi: NA
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.00
markerseek_score: 23.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE_p1	AGTCTTATGTTTTGATTTCTATGTGA	ACTATGGCTCGTCAATAATCT	26.9	38.1	55.2	54.2	482 bp	0.333333	13.3
ndhE_p2	GTCTTATGTTTTGATTTCTATGTGA	ACTATGGCTCGTCAATAATCT	28.0	38.1	53.9	54.2	481 bp	0.333333	13.3
ndhE_p3	AGTCTTATGTTTTGATTTCTATGTG	ACTATGGCTCGTCAATAATCT	28.0	38.1	53.9	54.2	482 bp	0.333333	13.3
ndhE_p4	AAGTCTTATGTTTTGATTTCTATGTGA	ACTATGGCTCGTCAATAATCT	25.9	38.1	55.9	54.2	483 bp	0.333333	13.3
ndhE_p5	AGTCTTATGTTTTGATTTCTATGTGAA	ACTATGGCTCGTCAATAATCT	25.9	38.1	55.9	54.2	482 bp	0.333333	13.3