Hotspot

trnN-GUU-trnR-ACG

IRa · 130050-130616 · 567 bp

Back to Tipularia

Pi 0.0329

MarkerSeek score 71.50

Primer available yes

Label trnN-GUU-trnR-ACG

Feature Metadata

label_name: trnN-GUU-trnR-ACG
feature_type: igs
region: IRa
length_bp: 567
variable_sites: 24
pi: 0.0329
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 71.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-trnR-ACG_p1	CCGTTGTTCCTCAGTAGCTC	CAGAGGATTAGAGCACGTGG	55.0	55.0	58.0	57.8	495-716 bp	1.0	85.3
trnN-GUU-trnR-ACG_p2	CCGTTGTTCCTCAGTAGCTC	TTTGAAGGTACTCCCGGGAA	55.0	50.0	58.0	58.3	548-769 bp	1.0	84.6
trnN-GUU-trnR-ACG_p3	CCGTTGTTCCTCAGTAGCTC	TTTTGAAGGTACTCCCGGGA	55.0	50.0	58.0	58.3	549-770 bp	1.0	84.6
trnN-GUU-trnR-ACG_p4	GCTGTTAACTGACTGGTCGT	CAGAGGATTAGAGCACGTGG	50.0	55.0	57.8	57.8	459-680 bp	1.0	84.3
trnN-GUU-trnR-ACG_p5	TGGTCGTAGGTTCGAATCCT	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.2	57.8	446-667 bp	1.0	84.3