Hotspot

rpl32-trnL-UAG

SSC · 113906-114546 · 641 bp

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Pi 0.035

MarkerSeek score 69.90

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 641
variable_sites: 32
pi: 0.035
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TCCACCGGACAGTCAAAAAG	GTCCCGAAGAAATCCAACGA	50.0	50.0	58.0	57.9	1090-1099 bp	1.0	83.7
rpl32-trnL-UAG_p2	TCCACCGGACAGTCAAAAAG	TGGCGAAACCTTGGGTAAAA	50.0	45.0	58.0	57.9	1237-1246 bp	1.0	83.4
rpl32-trnL-UAG_p3	TCCACCGGACAGTCAAAAAG	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.0	57.9	739-748 bp	1.0	83.4
rpl32-trnL-UAG_p4	TCCACCGGACAGTCAAAAAG	ATCCGCATAAAAGAGCTGCA	50.0	45.0	58.0	58.0	1357-1366 bp	1.0	83.3
rpl32-trnL-UAG_p5	TCCACCGGACAGTCAAAAAG	TCCGCATAAAAGAGCTGCAT	50.0	45.0	58.0	58.0	1356-1365 bp	1.0	83.3