Hotspot

trnH-GUG-rpl2

IRb · 85057-85099 · 43 bp

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Pi 0.062

MarkerSeek score 84.70

Primer available yes

Label trnH-GUG-rpl2

Feature Metadata

label_name: trnH-GUG-rpl2
feature_type: igs
region: IRb
length_bp: 43
variable_sites: 4
pi: 0.062
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 84.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-rpl2_p1	TGGATCAAGGCAGTGGATTG	GTTATCCTGCGCTTGGAAGA	50.0	50.0	57.9	58.0	179 bp	1.0	80.5
trnH-GUG-rpl2_p2	GGATCAAGGCAGTGGATTGT	GTTATCCTGCGCTTGGAAGA	50.0	50.0	57.9	58.0	178 bp	1.0	80.5
trnH-GUG-rpl2_p3	GTGGATCAAGGCAGTGGATT	GTTATCCTGCGCTTGGAAGA	50.0	50.0	57.9	58.0	180 bp	1.0	80.5
trnH-GUG-rpl2_p4	CGGATGTAGCCAAGTGGATC	GTTATCCTGCGCTTGGAAGA	55.0	50.0	58.1	58.0	193 bp	1.0	80.4
trnH-GUG-rpl2_p5	CGGATGTAGCCAAGTGGATC	CACAACCCCTTGGGGTTATC	55.0	55.0	58.1	58.2	207 bp	1.0	79.3