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rps3-rpl22

LSC · 83959-84019 · 61 bp

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Pi 0.0984

MarkerSeek score 74.40

Primer available yes

Label rps3-rpl22

Feature Metadata

label_name: rps3-rpl22
feature_type: igs
region: LSC
length_bp: 61
variable_sites: 9
pi: 0.0984
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rpl22_p1	CGATGTTGAGTCTCCGGTTT	TTATCATTCATTGGCGGGGG	50.0	50.0	57.9	58.0	793-798 bp	1.0	82.2
rps3-rpl22_p2	CGATGTTGAGTCTCCGGTTT	TTCGCGGACGTTCTTATGAG	50.0	50.0	57.9	58.1	670-675 bp	1.0	81.8
rps3-rpl22_p3	ACGTGCAATCTCTTTTCCGT	TTATCATTCATTGGCGGGGG	45.0	50.0	58.1	58.0	981-986 bp	1.0	81.4
rps3-rpl22_p4	TCGACACGTGCAATCTCTTT	TTATCATTCATTGGCGGGGG	45.0	50.0	57.9	58.0	986-991 bp	1.0	81.3
rps3-rpl22_p5	TTCGACACGTGCAATCTCTT	TTATCATTCATTGGCGGGGG	45.0	50.0	57.9	58.0	987-992 bp	1.0	81.3