Hotspot

rps11-rpl36

LSC · 79725-79894 · 170 bp

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Pi 0.0685

MarkerSeek score 74.30

Primer available yes

Label rps11-rpl36

Feature Metadata

label_name: rps11-rpl36
feature_type: igs
region: LSC
length_bp: 170
variable_sites: 15
pi: 0.0685
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 74.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11-rpl36_p1	ATTTTTGCTATAGGTTTTGTCA	GGCGAGGGCGAATTATAGTC	27.3	55.0	52.3	58.3	252-261 bp	1.0	48.7
rps11-rpl36_p2	TTTTTGCTATAGGTTTTGTCAT	GGCGAGGGCGAATTATAGTC	27.3	55.0	52.3	58.3	251-260 bp	1.0	48.7
rps11-rpl36_p3	TTTTTGCTATAGGTTTTGTCAT	GCGAGGGCGAATTATAGTCA	27.3	50.0	52.3	57.6	250-259 bp	1.0	48.7
rps11-rpl36_p4	ATTTTTGCTATAGGTTTTGTCA	ATGTCGCTTGATTCGTAGGC	27.3	50.0	52.3	58.4	269-278 bp	1.0	48.4
rps11-rpl36_p5	TTTTTGCTATAGGTTTTGTCAT	ATGTCGCTTGATTCGTAGGC	27.3	50.0	52.3	58.4	268-277 bp	1.0	48.4